84-1194-87　Transglutaminase II (TGase II, Transglutaminase 2, TG2, TGase 2, TGase2, TGase-2, TGM2, G-Protein alpha Subunit Gh Class, G-alpha-h, G[a]h, Gh Class G alpha h, Guanine Nucleotide Binding Protein H Polypeptide, GNAH, Protein Glutamine gamma Glutamyltransferase 2, Tissue Transglutaminase, Tissue-type Transglutaminase, Transglutaminase C, TGase C, TGase-C, TG(C), TGC, Transglutaminase H, TGase H, ...　T8260-03H

特徴

• Transglutaminase 2 is historically a member of the transglutaminase family that catalyzes a calcium-dependent acyl transfer reaction between peptide bound glutamine and a suitable primary amine.The protein is known to participate in all-trans-retinoic acid mediated signaling events as a G-protein and transamidate targets such as RhoA and the retinoblastoma gene product.
• The action of transglutaminase 2 stabilizes proteins by conferring mechanical resistance and protection from proteolysis.
• The protein is expressed intracellularly and secreted on the cell surface where it interacts with proteins in the extracellular matrix and aids in anchoring cells to basement membranes.
• Recently, transglutaminase 2 has been identified as an important factor that interacts with fibronectin in the extracellular matrix at the cell membrane and provides survival signals and inhibits anoikis in fibroblasts.
• The protein is expressed in human endothelial cells.
• The human transglutaminase 2 gene is localized in the chromosomal region 20q12.
• Transcript variants encoding two different isoforms have been found for this gene.
• Deficiency of the protein results in early-onset diabetes type 2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:BxPC-3 cell lysaste
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.