TRAM, or TRIF-related adaptor molecule, plays an essential role in the MyD88-independent signaling of TLR4.
The functional absence of TRAM in vascular endothelial cells restricts TLR4 signaling to MyD88-dependent pathway.
Overexpression of TRAM cDNA in endothelial cells reconstituted LPS-induced Trif-dependent NF-êB activation and cxcl10 promoter activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Lung Tissue Lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human TRAM. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: mouse, rat, canine, equine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa87-103 (EDDTDEALRVQNLLQDD) of human TRAM.
