The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain.
Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1).
The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2).
TLRs characterized so far activate the MyD88:interleukin-1 receptor-associated kinase (IRAK) signaling pathway.
Ten human homologs of TLRs (TLR1-10) have been described.
The aa sequence of human TLR6 is most similar to hTLR1 with 69% identity at the aa level.
Human TLR6 consists of a predicted 807aa with a molecular weight of approximately 92kD (3).
Human and mouse TLR6 share an aa identity of 73%.
TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK).
The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6.
It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4).
TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3).
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity：Recognizes human TLR6.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa25-39 (RIQFSDGNEFAVDKS) of human TLR6.
