The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain.
Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1).
The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2).
Ten human homologs of TLRs (TLR1-10) have been described (3).
TLR2 is differentially expressed in human cells.
TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers.
CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2.
The expression of TLR2 on different cell types are regulated by different immune response modifiers.
For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Bronchial epithelial cells (UBH16E), THP-1, or transfected human TLR2 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. Preservative free.
Specificity：Recognizes human TLR2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptides corresponding to aa180-196, 353-370, and 473-489 of human TLR2.
