TIM-1, also known as HAVcr1 (C Hepatitis A virus cellular receptor 1) and KIM-1 (Kidney Injury Molecule 1), is a 60-100kD member of the TIM family of immune regulating adhesion proteins.
The mature molecule is a type I transmembrane glycoprotein that contains a 214aa extracellular domain (ECD) and a 51aa cytoplasmic tail.
The ECD contains one Ig-like V-type domain, a Ser/Thr-rich mucin region, and multiple glycosylation sites.
TIM-1 is involved in the development of Th2-biased immune responses, and the presence of particular TIM-1 mutations correlates with the susceptibility to allergic disease.
TIM-1 binds TIM-4 and serves as the receptor for the hepatitis A virus.
It is also upregulated in renal epithelial cells following tissue injury.
The extracellular domain of TIM-1 can be shed and serves as an indicator of kidney tubule damage.
Within the ECD, rat TIM-1 shares 81% and 51% aa sequence identity with mouse and human TIM-1, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human or mouse brain tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human HAVCR1/TIM-1. Species Crossreactivity: mouse. Species sequence homology: monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide (SPLYSYTTDGNDTVT) corresponding to aa248-262 of human NP_036338 and aa84-98 of human EAW61615.1.
