TRH-R is a 398aa integral membrane protein with six transmembrane domains and belongs to G-protein coupled receptor 1 family.
It acts as a receptor for the signaling hormone TRH and activates the inositol phospholipid-calcium-protein kinase C transduction pathway upon binding with TRH.
Reports suggest that TRH-R is a neurotransmitter and neuro regulator that controls dimerization of the TRH receptor and potentiates hormone-induced receptor trafficking.
It is known to widely express in the thyrotroph cells of the anterior pituitary.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human TRH-R. Species Crossreactivity: chimpanzee, canine, monkey. Species sequence homology: mouse, rat, bovine, opossum.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Mk Mo
Immunogen：Synthetic peptide corresponding to aa350-400 of human TRH-R.
