TAK1 was shown to participate in regulation of transcription by transforming growth factor-beta (TGF-beta).
TAK1 is stimulated in response to TGF-beta and bone morphogenetic protein.
These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF-beta superfamily members.
TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF-b activated kinase 1) MAPKKK in TGF-b signal transduction.
TAB1-induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1.
TNF-a activated endogenous TAK1, and the kinase-negative TAK1 acted as a dominant negative inhibitor against TNF-a-induced NF-kB activation.
TAK1 was suggested to act as a regulatory kinase of IKKs.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:NIH 3T3
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human TAK1/MAP3K7 (TGF-beta-activated kinase 1). Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa19-35 and 564-579 of human TAK1 (accession number BAA25025).
