TBKBP1 is an adaptor protein belonging to the fibrillar collagen family, consisting of trimers of identical alpha 1 chains which are linked to each other by interchain disulfide bonds.
It is essential for signal transduction during viral infection and plays a major role in the NF-kB pathway.
TBKBP1 is thus required for efficient IRF (interferon regulatory factor) activation by Sendai virus.
The protein binds TBK1 (TANK-binding kinase) and IKKi (inhibitor of nuclear factor B kinase, inducible) via a newly identified TBK1/IKKi-binding domain (TBD), which it shares with TANK and NAP1.
Binding to kinases via TBD is essential for IRF3 (interferon regulatory factor 3) activation which controls the expression of antiviral genes during infection.
The protein has a ubiquitous expression with highest levels in ovary, followed by testis and brain.
Human TBKBP1 gene is mapped to chromosome 17q21.32.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-4.ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Brain
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human TBKBP1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa300-350 of human TBKBP1.
