ST2 is a membrane protein belonging to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain.
ST2, also known as IL1RL1, is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3:ERK1 and/or MAPK1:ERK2, MAPK14, and MAPK8.
Reports also suggest its possible role in helper T-cell function as well as in cell growth.
Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form.
ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease.
Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 4-5ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Kidney
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human ST2/IL1RL1. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, equine, mouse, orangutan, rhesus monkey.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aaa25-65 of human ST2 protein.
