Spermatogenic leucine zipper protein 1 is a member of the basic helix-loop-helix (bHLH) family of transcription factors which is expressed specifically in the testis and epididymis.
Studies show that SPZ1 acts downstream of mitogen-activated protein kinase (MAPK), an extracellular signal-regulated kinase 1:2 to up-regulate cell proliferation and tumorigenesis.
Also, through an interaction with proliferating cell nuclear antigen (PCNA) promoter, SPZ1 induced cell proliferation concomitant with an increase in PCNA gene expression.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Testis
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human SPZ1. Species Crossreactivity: chimpanzee, mouse, rat. Species sequence homology: canine, rhesus monkey.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa40-90 of human SPZ1.
