Sphingosine 1-phosphate receptor Edg8 is a multi-pass membrane protein belonging to the G-protein coupled receptor 1 family and coupled to both the G (i:0) alpha and G (12) subclass of heteromeric G-proteins.
Edg8 acts as a receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) as well as for lysolipid mediator lysophosphatidic acid (LPA) and gives diverse physiological effect on most types of cells and tissues.
Edg8 may also play a regulatory role in the transformation of radial glial cells into astrocytes and affect proliferative activity of these cells.
It is widely expressed in white matter tracts of brain, spleen, peripheral blood leukocytes, placenta, lung, aorta, and fetal spleen.
Over expression of Edg8 leads to a lymphoproliferative disorder often associated with the autoimmune disease known as leukemic large granular lymphocyte (LGL).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Sphingolipid Receptor Edg8/S1P5. Species Crossreactivity: canine, mouse, monkey, rat. Species sequence homology: opossum, bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-50 of human Edg8.
