Sorcin is a 198aa, soluble, resistance-related, calcium-binding cytosolic protein belonging to the penta-EF hand (PEF) family of calcium binding proteins and contains 4 EF-hand calcium-binding motifs.
Human Sorcin is known to share 95% sequence identity with hamster Sorcin.
It is known to associate with the sarcolemmal proteins Annexin VII, cardiac ryanodine receptors and L-type Ca2+ channels and thus influence the intracellular Ca (2+)-homeostasis.
It is also important in the regulation of intracellular Ca2+ cycling and Ca2+ influx pathways in the heart and skeletal muscle during excitation-contraction.
It is overproduced in many multi-drug resistance (MDR) cells and regulates cell apoptosis pathways, thus acting as a new MDR marker for prognosis of acute myeloid leukemia (AML) and a good target for anti-MDR drug development.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human Sorcin. Species Crossreactivity: chimpanzee, bovine, canine, equine, monkey, mouse. Species sequence homology: chicken, rat, xenopus, opossum.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Eq Hu Mk Mo
Immunogen：Synthetic peptide corresponding to aa50-100 of human Sorcin.
