Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus.
It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection.
B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation.
It consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail.
B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes Smallpox B5R. Species Crossreactivity: camel, human. Species sequence homology: bovine, monkey, rabbit.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa200-214 (GSDVDKKDTHGNTPF) of the B5R.
