SMADs are members of the MAD-related family of molecules.
MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.
SMADs can be divided into receptor-regulated SMADs (R-SMADs/ SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD/ SMAD4), and inhibitory SMADs (I-SMADs/ SMAD6 and SMAD7).
SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them.
SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
Human SMAD1 is a 465aa protein; GenBank Accession No.
AAP36050.1.
Human SMAD2 is a 467aa protein; GenBank Accession No.
AAC51918.1Human SMAD3 is a 425aa protein; GenBank Accession No.
NP_005893.1Human SMAD4 is a 552aa protein GenBank Accession No.
NP_005350.1.
Human SMAD5 is a 465aa protein; GenBank Accession No.
AAC50791.1.Human SMAD6 is a 496aa protein; GenBank Accession No.
AAH12986.1 Human SMAD7 is a 426aa protein; GenBank Accession No.
AAB81354.1Mouse SMAD8 is a 430aa protein; GenBank Accession No.
AAN85445.1Human SMAD9 is a 430aa protein; GenBank Accession No.
NP_005896.1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:A549, HepG2
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. Preservative free.
Specificity：Recognizes human SMAD7. May cross-react with SMAD6 depending on cell or tissue-specific expression. Species Crossreactivity: mouse and rat. Species sequence homology: chimpanzee, canine, pufferfish, xenopus, zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Two synthetic peptides corresponding to aa12-29 (RLWRSRAPGGEDEEEGAG) and aa36-50 (ELRGEGATDSRAHGA) of human SMAD7.
