RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain.
It is essential for membranous and endochondral bone formation.
It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression.
The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex.
It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression.
Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Saos-2 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human RUNX2/AML-3. Species Crossreactivity: chicken, chimpanzee, equine, monkey, mouse. Species sequence homology: rat, pufferfish, zebrafish, xenopus.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Eq Hu Mk Mo
Immunogen：Synthetic peptide (NPQGQSQITDPRQAQS) corresponding to aa264-279 of human RUNX2.
