RIP3 is a novel member of RIP Ser/Thr kinase family.
It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex.
RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction.
RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling.
It is recruited to the TNFR1 signaling complex in a RIP-dependent manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation.
RIP3 negatively regulates the TLR3-Trif−mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1.
The human RIP3 gene is localized in the chromosomal region 14q11.2.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human RIP3. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa480-518 of human RIP3.
