GRAIL is a type I transmembrane protein that localizes to the endocytic pathway and contains a PA (protease associated) domain and a RING-type zinc finger domain.
It is a ubiquitin-protein isopeptide ligase (E3) necessary for the induction of CD4(+) T cell anergy in vivo and induced expression of this protein was observed in anergic CD4 (+) T cells, which suggested a role in the induction of anergic phenotype.
It is differentially expressed in naturally occurring and peripherally induced CD25 (+) T regulatory cells and the expression is linked to the conversion of these cells to a regulatory phenotype.
Expression of GRAIL in retrovirally transduced T cell hybridomas limits activation-induced IL-2 and IL-4 cytokine production.
It also functions in the patterning of the dorsal ectoderm and sensitizes ectoderm to respond to neural-inducing signals.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat spleen lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human RNF128. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat. Species sequence homology: chicken, opossum.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Eq Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to a (IFYSARRLRNARAQSRKQRQLKADAKK) portion of aa227-253 of human GRAIL, GenBank no. ref|NP_919445.1|.
