Plays important roles in the production of 11-cis retinal and in visual pigment regeneration.
The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester.
The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol.
The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.
The enzymatic activity is linearly dependent of the expression levels and membrane association.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100-1:2000Peptide ELISA: detection limit 1:32,000Optimal dilutions to be determined by the researcher.
Positive Control:Rat Retina lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Specificity：Recognizes rat RPE65 at ~60kD. Species Crossreactivity: human and canine
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Rt
Immunogen：Synthetic peptide corresponding to C-EVKKNARKAPQPE, from the internal region of human RPE65 (NP_000320.1).
