84-1193-84　［取扱停止］Ras, R (R-Ras, RRAS, Oncogene RRAS, p23, Ras-related Protein, Ras-related Protein R-Ras, Related RAS Viral (r-Ras) Oncogene Homolog) 50ul　R1200-10B

特徴

• R-Ras is a member of the Ras superfamily of GTPases.
• Ras proteins function as signaling focal points that are activated by convergent signaling pathways intiated by extracellular stimuli (reviewed in Mitin et al, 2005).
• Activated Ras proteins in turn regulate a diversity of downstream cytoplasmic signaling cascades.
• Many members of the Ras superfamily are implicated in the regulation of hematopoietic cells, with roles in growth, survival, differentiation, cytokine production, chemotaxis, vesicle-trafficking, and phagocytosis.
• The Ras superfamily of proteins includes over 150 small GTPases (distinguished from the large, heterotrimeric GTPases, the G-proteins).
• It comprises six subfamilies, the Ras, Rho, Ran, Rab, Arf, and Kir:Rem:Rad subfamilies.
• They exhibit considerable overall aa identities, especially in the regions interacting with the guanine nucleotide exchange factors that catalyze their activation.
• Recognizes R-Ras; human R-Ras is a 218aa protein.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Heart colon spleen, CA cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.