ROR-gamma is a 560aa novel DNA-binding transcription factor belonging to the ROR:RZR orphan nuclear receptor subfamily.
The specific functions of this protein are not known.
However, reports suggest that this protein is a ligand activated transcription factor and may be essential for lymphoid organogenesis (lymph nodes and Peyer's patches) and may play an important regulatory role in controlling apoptosis during thymopoiesis.
ROR-gamma inhibits the expression of Fas ligand and IL2 and directs the differentiation program of proinflammatory IL-17+ T helper cells.
ROR-gamma shares 50-51% sequence identity with RORA and RORB and is 88% identical to mouse ROR-gamma.
It is highly expressed in skeletal muscle and at lower levels in various other tissues.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat, NIH 3T3 or RAW cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Retinoid-Related Orphan Receptor Gamma. Species Crossreactivity: chimpanzee. Species sequence homology: mouse, rat, canine, bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1-50 of human RORg.
