RAP2A is a signaling protein involved in small GTPase mediated signal transduction activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2, in a cAMP-dependent manner, and GFR.
This membrane protein belongs to the small GTPase superfamily and Ras family and contains domains required for GTP binding and a Ras-like C-terminal motif for post-translational lipid binding and subsequent anchoring to the plasma membrane.
Apart from having a possible role in membrane shedding, RAP2A also functions as a slowly responding molecular switch in the RAP1A signaling cascade and also interacts with ARHGAP29, the putative specific effector of RAP2A.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:MCF7 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human RAP2A. Species Crossreactivity: chicken, canine, equine, money, mouse, opossum, xenopus. Species sequence homology: chimpanzee, pufferfish, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Ch Eq Hu Mk Mo Xe
Immunogen：Synthetic peptide corresponding to aa140-190 of human RAP2A.
