Rad17, also known as 'cell cycle checkpoint protein RAD17,' is a nuclear protein belonging to the Rad17/RAD24 family and necessary for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage.
Rad17 is known to have a weak ATPase activity and is required for binding to chromatin and also participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation.
It may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Rad17 is a part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5.
Four isoforms have been reported by X-ray irradiation.
Overexpression of this protein is seen in various cancer cell lines and in colon carcinoma.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.025-1ug/mlImmunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human RAD17, phosphorylated at Ser645. Species Crossreactivity: chimpanzee, mouse, orangutan and Rhesus monkey.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to a portion of human RAD17 containing a phosphorylated serine residue at position 645. Species Sequence Homology: Rhesus monkey, chimpanzee, orangutan; 100%, equine, bovine, mouse; 92%, canine, rat; 85%
