Rab7 is a 207aa transport carrier, belonging to the Rab family of RAS-related GTP-binding proteins with 4 GTP:GDP-binding sites and terminates in sequences such as cys-X-cys, cys-cys or a closely related sequence.
Rab7 located in specific intracellular compartments is known to be an important regulator of vesicular transport of proteins from early to late endosomes and lysosome biogenesis.
Rab7 accelerates thyroid hormone production controlling aggregation and fusion of late lysosomes.
It is ubiquitously expressed in most of the tissues and plays an essential role in maintenance of the perinuclear lysosome compartment.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human Rab7. Species Crossreactivity: chicken, chimpanzee, bovine, canine, monkey, rat. Species sequence homology: zebrafish, xenopus.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Ch Hu Mk Rt
Immunogen：Synthetic peptide corresponding to aa90-140 of human Rab7 protein.
