ASC2/POP1 is a PAAD domain only protein originally identified in a bioinformatics screen aimed at understanding molecular apoptosis mechanisms (Pawtokski et al, 2001; reviewed in Fabiola et al, 2006, and Mariathasan and Vuvic, 2003.).
ASC2/POP1 has high aa sequence homology with ASC (64%), hence it was originally termed ASC2.
The PAAD (also known as PYRIN) domain is a conserved sequence motif identified in more than 35 human proteins with putative functions in apoptosis and inflammatory signaling pathways.
PAAD was named after the protein families from which it was discovered: pyrin, AIM (absent-in-melanoma), ASC [apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD], and death-domain (DD)-like.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:2000Immunohistochemistry (Paraffin): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Positive Control:Spleen
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide
Specificity：Recognizes human ASC2. Species Crossreactivity: gerbil, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to full-length human ASC2.
