84-1193-71　PKN1 (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, AK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, PAK1, PKN, PRK1, PRKCL1, Serine-threonine Protein Kinase N, ) 50ul　P9103-72Q3

特徴

• Protein Kinase 1 (PKN) belongs to the protein kinase C superfamily.
• This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway.
• This kinase can be activated by phospholipids and by limited proteolysis.
• The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton.
• The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
• Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Spleen, lymphoid, CA cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.