Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells.
PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries.
It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues.
Only normal colon and placenta, which are known to be mitotically active express PLK.
PLK expression is restricted to proliferating cells.
In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified.
PLK1 is the most studied isoform.
The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B.
PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Positive Control:Human HCT-116 or mouse NIH 3T3 cell line
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human PLK1. Species Crossreactivity: bovine, canine, monkey, mouse, porcine, rat, rhesus monkey. Species sequence homology: c elegans, xenopus, zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Hu Mk Mo Po Rt
Immunogen：Synthetic peptide corresponding to aa100-200 of human PLK1.
