PACSIN2, a 486aa phosphoprotein is a novel member of the PACSIN family of cytoplasmic adapter proteins.
It is an isoform of PACSIN with a CDC15 N-terminal domain, a C-terminal SH3 domain, 3 conserved regions specific to the PACSIN family, and 3 asn-pro-phe (NPF) motifs, which potentially bind to EH domains.
This protein is known to display a ubiquitous expression pattern with the highest levels of expression in brain.
Reports suggest that PACSIN2 participates in the organization of the actin cytoskeleton, the regulation of vesicular traffic.
It also participates in endocytic internalization and endocytic receptor recycling.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.02-1ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human PACSIN2. Species Crossreactivity: chimpanzee, xenopus. Species sequence homology: mouse, rat, canine, bovine, chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa400-450 of human PACSIN2.
