PACS-1 (CRA_a) is a 963aa rich regulatory protein involved in protein trafficking and belongs to the PACS family.
A coat protein involved in controlling the correct subcellular localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs, PACS-1 is also known to be involved in HIV-1 Nef-mediated removal of MHC-I from the cell surface to the TGN, thus enabling HIV-1 to escape immune surveillance.
PACS-1 also controls the endosome to Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin coated membrane pits.
Usually seen in golgi apparatus, trans-Golgi network and localized in the paranuclear region, it is ubiquitously expressed in most of the tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.05-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human uterus lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human PACS-1. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, opossum. Species sequence homology: chicken, zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Eq Hu Mo
Immunogen：Synthetic peptide corresponding to aa300-350 of human PACS-1.
