DUBA, also known as OTU domain-containing protein 5, is a de-ubiquitinating enzyme belonging to the OTU5 family and DUB subfamily characterized by an ovarian tumor (OTU) domain.
DUBA is a protease that specifically cleaves ubiquitin linkages, negating the action of ubiquitin ligases.
This de-ubiquitinating activity is directed towards 'Lys-63'-linked polyubiquitin chains and acts via TRAF3 de-ubiquitination and subsequent suppression of type I interferon (IFN) production.
DUBA acts as a negative regulator of the innate immune system.
It is generally inhibited by N-ethyl-maleimide (NEM).
Expression of this enzyme is seen in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.
DUBA is induced by bacterial lipopolysaccharides (LPS) in bone marrow-derived macrophages.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 3-5ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Liver
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human DUBA. Species Crossreactivity: chimpnzee, mouse, rat. Species sequence homology: bovine, canine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa550-600 of human DUBA.
