OLFM4 is a 510aa extracellular matrix glycoprotein belonging to the olfactomedin-related protein family and found to be selectively expressed in inflammed colonic epithelium.
It has one signal peptide, six N-linked glycosylation motif and forms disulfide-bonded multimers.
OLFM4 a transcription factor may have a matrix-related function in differentiation.
Constitutively expressed OLFM4 in cancer cells binds to the potent apoptosis inducer GRIM-19 and has a novel antiapoptotic action and hence might serve as a diagnostic marker for human cancers.
Reports suggest that OLFM4 promotes proliferation of PANC-1 cells by favoring transition from the S to G2:M phase.
Potential interaction of OLFM4 with endogenous cell surface lectins and cadherins may mediate cell adhesion.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Olfactomedin 4. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, canine, monkey, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 400-419 (YVYNDGYLLNYDLSVLQKPQ) of human Olfactomedin 4, Gen Bank. gb|AAH47740.1|
