Nucleoporin Nup35, or Mitotic phosphoprotein 44, is a nuclear protein known to be present on the nuclear pore complex and is tightly associated with the nuclear membrane and lamina.
This RNA-associated protein belongs to the Nup53 family and has a MPPN (mitotic phosphoprotein N' end) domain.
It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins.
Though the exact function of NUP35 is yet to be revealed, it may function as a component of the nuclear pore complex (NPC) which are collectively referred to as nucleoporins (NUPs).
It also may play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors, and may also play an important part in the association of MAD1, a transcriptional repressor with the NPC.
NUP35 is widely expressed in most of the tissues.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Liver lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human NUP35. Species Crossreactivity: chimpanzee, canine, bovine, monkey, mouse. Species sequence homology: chicken, opossum, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Hu Mk Mo
Immunogen：Synthetic peptide corresponding to aa280-330 of human NUP35.
