84-1193-35　Noxa (Phorbol-12-Myristate-13-Acetate-Induced Protein 1, PMA-induced Protein 1, Immediate-early-response Protein APR, Protein Noxa, Pmaip1) 100ug　N5376-82A

特徴

• Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage).
• The expression of the Noxa gene involves direct activation of its promoter by p53.
• Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes.
• Noxa cDNA codes for a 103aa protein.
• The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1.
• Oda et al have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis.
• Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry: ParaffinOptimal dilutions to be determined by the researcher.
• Positive Control:Human, mouse or rat thymus tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.