NKX2-5 is a transcription factor protein which plays a crucial role in cardiac development and differentiation.
It is a low molecular weight protein of 35-38kD depending on phosphorylation status.
Expression is mainly restricted to the heart.
But during embryogenesis NKX2-5 expression has also been detected in spleen-precursor cells.
Over expression of NKX2-5 resulted in expression of neuronal markers in C2C12 murine myogenic cells.
Functionally it is involved in differentiation processes in heart development and in homeostasis and survival of cardiac myocytes as well maintains the initial muscle differentiation paradigm.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human NKX2-5. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: mouse, bovine, equine, canine, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa300-324 of human NKX2-5.
