NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation.
The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product.
N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
Applications:Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100-1:2000Immunofluorescence (IC): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human NF-kB (p65). Species Crossreactivity: chimpanzee and New World monkey
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to aa1-50 of human p65 protein.Species Sequence Homology: canine, mouse and rat
