84-1193-21　NANOG (Homeobox Protein NANOG, Homeobox Transcription Factor Nanog, hNanog) 100ug　N0019-01U

特徴

• NANOG is a unique variant of NK2 family of transcription factors.
• It is a human ortholog of mouse Nanog and consists of a highly conserved homeodomain and Trp-rich repeats.
• It is specifically expressed by embryonic stem cells and is crucial for second embryonic lineage determination.
• It works in concert with OCT-4 and SOX-2 and plays a central role in regulating pluripotency and self-renewal of ICM and ES cells.
• It functions independent of LIF:gp130:Stat3 pathway and prevents differentiation of ES cells to extraembryonic endoderm and trophectoderm lineages.
• Its expression has also been detected in embryonic carcinoma cells and germ line tumors indicating a therapeutic intervention.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:HeLa (human), NIH 3T3 (mouse)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.