NALP10, a member of the NALP protein family, is reported to have a regulatory role in the innate immune system and forms a large signal-induced multiprotein complex, the inflammasome, which activates the pro-inflammatory caspases, caspase-1 and caspase-5.
Unlike other members of the family NALP10 lacks the LRR (leucine rich repeat) region but contains aDPIN domain and a NACHT domain.
Immunoprecipitation reports suggest that NALP10 interacted with ASC, CASP1, and IL-1B and inhibits NF-kB activation, CASP1/caspase-1-dependent IL-1B secretion and apoptosis induced by ASC (PYCARD) in a dose-dependent manner.
Expression of NALP10 is enhanced by stimulation with lipopolysaccharide.
Experiments with NALP10 suggest that it acts as a multifunctional negative regulator of inflammation and apoptosis.
Wide expression of NALP10 is reported with highest levels in heart, brain and skeletal muscle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa and RAW cell lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human NALP10. Species sequence homology: equine, rhesus monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa493-509 of human NALP10 REVQRLLEVKEQEGNDE protein.
