84-1193-17　NALP4 (NACHT-, LRR-and PYD-containing Protein 4, NACHT Leucine Rich Repeat and PYD Containing 4, CLR19.5, FLJ32126, Nucleotide Binding Oligomerization Domain Leucine Rich Repeat and Pyrin Domain Containing 4, NLR Family Pyrin Domain Containing 4, NLRP4, PAAD and NACHT Containing Protein 2, PAN2, PYRIN Containing APAF1-like Protein 4, PYPAF4, Ribonuclease Inhibitor 2, RNH2) 50ul　N0018-65B

特徴

• NALP4 is a 994aa cytoplasmic regulatory protein involved in inflammatory response.
• It belongs to the NLRP family and contains a PYD domain, eight LRR (leucine rich) repeats and a NACHT domain.
• Like other NALP proteins, NALP4 may also form multiprotein complexes called inflammasomes and thus may be involved in the activation of proinflammatory caspases and apoptosis.
• NALP4 is known to resemble Cryopyrin, a protein involved in hereditary hyperinflammation syndromes.
• Immunoblot and mutation analysis suggest that NALP4 inhibits the induction of NF-kB DNA-binding activity by the cytokine TNF and IL-1B through its PYD domain and also suppress IKKA activity via a mechanism that did not involve its PYD or LRR domain.
• It is widely expressed in most tissues including spleen, followed by placenta, lung, liver, kidney, pancreas, and thymus.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000Immunoprecipitation: 1:50-1:200Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
• Positive Control:Spleen, lymphoid, CA cell lines
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.