Mucin 13 is a 512aa hemopoietic transmembrane mucin belonging to a family of secreted and cell surface glycoproteins and may play a role in cell signaling.
It contains a serine/threonine-rich mucin repeat domain predicted to be heavily O-glycosylated, three cysteine-rich EGF-like domains, a SEA domain and also has sites for N-glycosylation and phosphorylation.
Activation of NF-kappa-B signaling pathway by the inflammatory cytokines TNF-alpha and TGF-beta leads to upregulation of Mucin 13.
It is a homodimer of beta subunits and is highly expressed in epithelial tissues, gastrointestinal and respiratory tracts, kidney, small intestine, appendix and stomach.
RT-PCR and immunoblot analysis supports the up regulation of Mucin 13 in gastric cancers and is usually present on the apical side of tubular glands in intestinal-type cancers and in the cytoplasm in diffuse cancers.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-0.5ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human Mucin 13. Species sequence homology: chimpanzee, monkey.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa480-530 of human Mucin 13.
