Metastasis suppressor protein 1 (MTSS1) [MIM, "missing in metastasis"], was first discovered as missing transcripts from metastatic bladder and prostate cancer cells.
MTSS1 is a 755aa protein, having multidomain and scaffolding function.
It has a proline-rich/SH3 binding region and a serine-rich region with EGF or PDGF/Src consensus sites.
It contains an actin-binding Wiskott-Aldrich syndrome protein homology-2 motif (WH2) at the C-terminus while the N-terminal IMD domain (IRSp53-MIM domain) interact with membranes and bundle actin filament.
The WH2 domain binds to actin monomers and plays an important role in actin cytoskeleton reorganization.
Recently, MIM was found to interact with cortactin via SH3 domains.
In addition, MTSS1 enhanced cortactin- and Arp2/3-dependent actin polymerization.
It is thus involved in cell motility and morphogenesis and also interacts with sonic hedgehog/Gli signaling in epidermal cells.
MTSS1 acts as an important regulator of cell growth and development and is known to express in many tissues, including spleen, thymus, prostate, testis, uterus, colon, and peripheral blood.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-4ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat testis lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human MTSS1. Species Crossreactivity: chimpanzee. Species sequence homology: mouse, rat, equine, canine, bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa670-720 of human MTSS1 protein.
