Human p44/42 MAP Kinase belongs to the family of signal transduction intermediates leading to cell proliferation or differentiation, known as MAPKs (mitogen-activated protein kinases).
It plays an important role in the signal transduction pathways that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals.Activation of the protein requires phosphorylation of both tyrosine and threonine residues by upstream dual-specific kinases.
Active MAPKs are responsible for the phosphorylation of a variety of effector proteins, including several transcription factors.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry (paraffin): 1:50-1:100Immunofluorescence/Immunocytochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Positive Control:NIH-3T3 cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAP Kinase p44/42, phosphorylated at Tyr204. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-Yp-V-A).
