MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000).
MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain.
It binds to Bcl10 through its Ig-like domains and cooperates with Bcl10-activate NF-kappaB.
MALT1 is thought to play an important role in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl10 (Ho et al., 2005).
Interaction between MALT1 and Bcl10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitin-conjugating enzyme UBC13.
Four recurrent chromosomal translocations have been described in non-Hodgkin B-cell lymphoma of the mucosa-associated lymphoid issue (MALT) type.
Two of them,t(14;18)(q32;q21) and t(11;18)(q21;q21), are found in 30% to 50% of extranodal MALT lymphomas.
The first translocation juxtaposes the MALT1 gene to the IgH promoter region, resulting in the deregulation of MALT1 expression, whereas the second translocation generates a fusion between MALT1 and the inhibitor of apoptosis gene API2 (cIAP2/HIAP1).
The other two much rarer translocations, t(1;14)(p22;q32)and t(1;2)(p22;p12), target Bcl10 gene on the short arm of chromosome 1.
Translocations involving Bcl10 and MALT1 may lead to elevated NF-kappaB activity in MALT B-cell lymphoma.
Two alternatively spliced transcript variants encoding different isoforms (a and b) have been described for the MALT1 gene.
Human MALT1 isoform a is an aa protein of 824aa, and human MALT1 isoform b is an aa protein of 813aa.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human MALT1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa43-57 (ELLDQAPEGRGWRRL) of human MALT1 (GenBank No. NP_776216.1).
