MafA is a 352aa containing transcription factor playing an important part in the regulation of the insulin gene transcription.
It belongs to the bZIP family and Maf subfamily with a bZIP domain and binds DNA as a homodimer.
Transcription factor MafA binds to RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene.
MafA is known to cooperate synergistically with NEUROD1 and PDX1 and is known to be up-regulated by glucose.
It is detected in nuclei of pancreas islet beta cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-0.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Liver lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human MafA. Species Crossreactivity: mouse, opossum, rat. Species sequence homology: chicken, bovine, canine, equine, rhesus monkey, xenopus, zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa300-350 of human MafA.
