c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors.
The leucine-zipper domain is involved in the interaction with LRPICD.
There are two forms of human c-maf mRNA, c-maf-long and c-maf-short.
It is identified in the genome of the acute transforming avian retrovirus AS42.
c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet , p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE).
It regulates Th2 differentiation and lineage-specific hematopoiesis.
It is a transcription factor for IL-10 gene expression in LPS-activated macrophages.
Chromosomal aberration involving maf is found in some forms of multiple myeloma.
It is expressed in myeloma cell lines and resting monocytes:macrophages.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human, mouse and rat brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human c-maf. Species Crossreactivity: bovine, mouse, rat. Species sequence homology: xenopus, chicken, opossum.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa75-90 (APSPGSGSEQKAHLED) of human c-maf, GenBank no. NP_001026974.1|.
