LSD1 is a flavin-containing novel nuclear protein having an N-terminal SWIRM domain and a C-terminal FAD-binding motif and an amine oxidase domain.
It functions as a transcriptional corepressor and catalyzes the flavin-dependent demethylation of Lys4 of histone3 resulting in the formation of methyl-free lysine and release of formaldehyde.
LSD1 has been found to be typically associated with CoREST and histone deacetylases 1 and 4 and participates in the silencing of endogenous neuron-specific genes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Brain lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human LSD1. Species Crossreactivity: canine, mouse, rat, rhesus monkey, zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ca Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to aa100-150 of human LSD1.
