84-1192-86　Lysyl Oxidase (LOX, Protein-lysine 6-oxidase) 100ul　L2623-11A

特徴

• Protein-lysine-6 oxidase; LOX (EC 1.4.3.13), a 30kD copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes.
• It plays a vital role in the formation and repair of ECM.
• Localized both in the cytosol and nucleus, it catalyzes the oxidation and deamination of epsilon-NH2 group of lysine and hydroxylysine residues to alpha-aminoadipic-delta-semialdehyde.
• This is the first step that initiates the covalent cross-linking of collagen and tropoelastin resulting in the formation of insoluble collagen and elastic fibers in the extracellular matrix.
• LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence.
• Deficiency of LOX is seen in certain carcinomas and 3X-linked recessive human connective tissue disorders—Cutis Laxa.
• Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500Immunohistochemistry (FFPE): 1:100Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.