Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells.
Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD).
Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules.
Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG).
Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.
Applications:Suitable for use in Confocal Microscopy, Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry (formalin fixed paraffin embedded): 5g/mlImmunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes mouse Langerin. Species sequence homology: rat.
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa26-44 of mouse Langerin (Genbank Accession No. CAC82936).
