Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily.
Interleukin-12 alpha chain precursor (IL-12A) is a 35kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities.
Reports suggest that Interleukin 12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK:lymphokine-activated Killer cells.
It stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells.
It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein.
The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A.
Along with EBI3 it forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 3-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human IL12A. Species Crossreactivity: primate
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of aa200-250 of p35/IL-12A. Species sequence homology: monkey and gorrila (100%), canine and mouse (95%)
