84-1192-64　IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI) 100ug　I2500-05E

特徴

• NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1).
• Synthesis of IkBa is autoregulated (2).
• IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6).
• External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers.
• NF-kB dimer subsequently translocates to the nucleus and activates target genes.
• Six members of IkB family members have been identified (1).
• One of the first genes induced following NF-kB activation is IkBa.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Jurkat
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.