Humanin is a novel 24aa polypeptide identified in Alzheimer's disease-affected brain.
It is 99% homologous to mitochondrial 16s ribosomal RNA and functions as a novel neuroprotective and cytoprotective factor.
It specifically inhibits neuronal cell death induced by beta-amyloids and Alzheimer's disease causing FAD genes by competitively inhibiting access of FPRL1 receptor to beta-amyloids.
It acts as an anti-apoptotic factor by binding to Bcl-2/Bax family of pro-apoptotic factors including Bax, Bak, Bid and BimEL and inhibits their pro-apoptotic activity.
It activates JAK/STAT signaling pathway and is regulated by TRIM11.
It interacts with IGFBP-3 and specifically blocks IGFBP-3 induced cell death in glioblastoma cells.
Humanin is also expressed in colon and testis.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000ELISA: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Recombinant protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity：Recognizes human Humanin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1-24 of human humanin. UniProt Accession: Q8IVG9
