HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family.
It is also called pregnancy-related serine protease.
HtrA3 has two isoforms (long and short), which is produced by alternative splicing.
Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibited the signaling of BMP-4, -2 and TGF-beta 1.
HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurred upstream of the cell surface receptor.
Applications:Suitable for use in Western Blot and Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity Chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human HtrA3. The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa250-300 of human HtrA3. Species sequence homology: feline (93%)
